CK2 Product Line

CK2 as Drug Target

CK2 plays a role in a number of cellular processes, including maintenance of cell viablity, protection of cells from apoptosis, cellular stress response. Its overexpression is associated with tumor development and CK2 has been shown to be involved in a variety of other diseases, such as glomerulonephritis, globozoospermia, inflammatory and autoimmune disease, cardiac hypertrophy. Hence, CK2 is considered a promising drug target.

CK2 Product Line

KinaseDetect offers the most complete CK2 product line. We provide all three subunits (alpha 1, alpha 2, beta), deletion mutants, holoenzymes containing either the alpha 1 or alpha 2 subunit as well as monoclonal antibodies against the catalytic and regulatory subunits.

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Related Literature

CK2 as Drug Target

Sandholt IS, Olsen BB, Guerra B, Issinger OG (2009) Resorufin: a lead for a new protein kinase CK2 inhibitor. Anticancer Drugs 20, 238-248. Download Article

Niefind K, Issinger OG (2008) Targeting Protein/Protein Interactions: Protein Kinase CK2 as a Prime Example for a Novel Concept. Screening, Trends in Drug Discovery 9, 18-21. Download Article

Guerra B, Issinger OG (2008) Protein Kinase CK2 in Human Diseases. Current Medicinal Chemistry, Volume 15, Number 19, in press. Download Abstract

Duncan JS, Litchfield DW (2008) Too much of a good thing: The role of protein kinase CK2 in tumorigenesis and prospects for therapeutic inhibition of CK2. Biochim Biophys Acta 1784, 33-47. Download Abstract

Hauck L et al. (2008) Protein kinase CK2 links extracellular growth factor signaling with the control of p27Kip1 stability in the heart. Nature Medicine 14, 315-324. Download Abstract

Yamada M et al (2005) Inhibition of protein kinase CK2 prevents the progression of glomerulomephritis. Proc Natl Acad Sci USA 102, 7736-41. Download Abstract

Xu X, Toselli PA, Russel LD, Seldin DC (1999) Globozoospermia in mice lacking the casein kinase II a´ catalytic subunit. Nature Genetics 23, 118-121. Download Abstract

CK2 subunits and holoenzymes: Biochemical characterization and crystallization

Olsen BB, Rasmussen T, Niefind K, Issinger OG. (2008) Biochemical characterization of CK2a and a´ paralogues and their derived holoenzymes: Evidence for the existence of a heterotrimeric CK2a´- holoenzyme forming trimeric complexes. Mol Cell Biochem, in press. Download

Raaf J, Issinger OG, Niefind K (2008) Insight from soft X-rays: the chlorine and sulphur sub.structures of a CK2alpha/DRB complex. Mol Cell Biochem, in press. Download Abstract

Raaf J, Klopffleisch K, Issinger OG, Niefind K (2008) The catalytic subunit of protein kinase CK2 structurally deviates from its maize homologue in complex with the nucleotide competitive inhibitor emodin. J Mol Biol, 377, 1-8. Download Abstract

Raaf J, Brunstein E, Issinger OG, Niefind K (2008) The CK2alpha/ CK2beta interface of human protein kinase CK2 harbors a binding pocket for small molecules. Chem Biol 15, 111-117. Download Abstract

Niefind K, Yde CW, Ermakova I, Issinger OG (2007) Evolved to be active: sulfate ions define substrate recognition sites of CK2alpha and emphasise its exceptional role within the CMGC family of eukaryotic protein kinases. J Mol Biol 370, 427-438. Download Abstract

Olsen BB, Boldyreff B, Niefind OG, Issinger OG (2006) Purification and characterization of the CK2alpha´-based holoenzyme, an isozyme of CK2alpha: a comparative analysis. Protein Expr Purifi 47, 651-661. Download Abstract

Rasmussen T, Skoeth IH, Jensen HH, Niefind K, Boldyreff B, Issinger OG (2005) Biochemical characterization of the recombinant human Drosophila homologues Timekeeper and Andante involved in the Drosophila circadian oscillator. Mol Cell Biochem 274, 151-161. Download Abstract

Ermakova I, Boldyreff B, Issinger B, Niefind K (2003) Crystal structure of a C-terminal deletion mutant of human protein kinase CK2 catalytic subunit. J Mol Biol 330, 925-934. Download Abstract

Niefind K, Guerra B, Ermakova I, Issinger OG (2001) Crystal structure of human protein kinase CK2: insights into basic properties of the CK2 holoenzyme. The EMBO J 20, 5320-31. Download Abstract

Grankowski N, Boldyreff B. Issinger OG (1991) Isolation and characterization of recombinant casein kinase II subunits alpha and beta from bacteria. Eur J Biochem 198, 25-30. Download Abstract

Antibodies against CK2 subunits

Schmidt-Spaniol I, Boldyreff B, Issinger OG (1992) Isolation and characterization of a monoclonal anti CK-2 alpha subunit antibody of the IgG1 subclass. Hybridoma 11, 53-59. Download Abstract

Nastainczyk W, Issinger OG, Guerra B (2003) Epitope analysis of the MAb 1AD9 antibody detection site in human protein kinase CK2alpha-subunit. Hybrid Hybridomics 22, 87-90. Download Abstract

Nastainczyk W, Schmidt-Spaniol I, Boldyreff B, Issinger OG (1995) Isolation and characterization of a monoclonal anti-protein kinase CK2beta-subunit antibody of the IgG class for the direct detection of CK2beta in tissue cultures of various mammalian species and human tumors. Hybridoma 14, 335-339. Download Abstract