CK2 Product Line

CK2 as Drug Target

CK2 plays a role in a number of cellular processes, including maintenance of cell viablity, protection of cells from apoptosis, cellular stress response. Its overexpression is associated with tumor development and CK2 has been shown to be involved in a variety of other diseases, such as glomerulonephritis, globozoospermia, inflammatory and autoimmune disease, cardiac hypertrophy. Hence, CK2 is considered a promising drug target.

CK2 Product Line

KinaseDetect offers the most complete CK2 product line. We provide all three subunits (alpha 1, alpha 2, beta), deletion mutants, holoenzymes containing either the alpha 1 or alpha 2 subunit as well as monoclonal antibodies against the catalytic and regulatory subunits.

To obtain prices and availability request further information.

Product	Product Information	Request Price or Further Information
CK2 alpha 1 (1-335), crystallization grade	Datasheet	Inquire
CK2 alpha 1 full length	Datasheet	Inquire
CK2 alpha, Zea mays, crystallization grade	Datasheet	Inquire
CK2 alpha 2	Datasheet	Inquire
CK2 beta	Datasheet	Inquire
CK2 beta mutants		Inquire
CK2 holoenzyme (alpha 1 1-335), crystallization grade	Datasheet	Inquire
CK2 holoenzyme (alpha 1 full length)		Inquire
CK2 holoenzyme (alpha 2)	Datasheet	Inquire
CK2 substrate peptide 1	Datasheet	Inquire
CK2 substrate peptide 2	Datasheet	Inquire
Anti CK2 alpha (1AD9)	Datasheet	Inquire
Anti CK2 beta (6D5)	Datasheet	Inquire

Download CK2 Product Line Brochure

Related Literature

CK2 as Drug Target

Kreutzer JN, Olsen BB, Lech K, Issinger OG, Guerra B (2011) Role of polyamines in determining the cellular response to chemotherapeutic agents: modulation of protein kinase CK2 expression and activity. Mol Cell Biochem, in press. Download

Poster on "Resorufin: A Lead for a New Protein Kinase CK2 Inhibitor" presented by KinaseDetect at Screening Europe 2009 in Berlin, Germany.

Sandholt IS, Olsen BB, Guerra B, Issinger OG (2009) Resorufin: a lead for a new protein kinase CK2 inhibitor. Anticancer Drugs 20, 238-248. Download Article

Niefind K, Issinger OG (2008) Targeting Protein/Protein Interactions: Protein Kinase CK2 as a Prime Example for a Novel Concept. Screening, Trends in Drug Discovery 9, 18-21. Download Article

Guerra B, Issinger OG (2008) Protein Kinase CK2 in Human Diseases. Current Medicinal Chemistry, Volume 15, Number 19, in press. Download Abstract

Duncan JS, Litchfield DW (2008) Too much of a good thing: The role of protein kinase CK2 in tumorigenesis and prospects for therapeutic inhibition of CK2. Biochim Biophys Acta 1784, 33-47. Download Abstract

Hauck L et al. (2008) Protein kinase CK2 links extracellular growth factor signaling with the control of p27Kip1 stability in the heart. Nature Medicine 14, 315-324. Download Abstract

Yamada M et al (2005) Inhibition of protein kinase CK2 prevents the progression of glomerulomephritis. Proc Natl Acad Sci USA 102, 7736-41. Download Abstract

Xu X, Toselli PA, Russel LD, Seldin DC (1999) Globozoospermia in mice lacking the casein kinase II a´ catalytic subunit. Nature Genetics 23, 118-121. Download Abstract

CK2 subunits and holoenzymes: Biochemical characterization and crystallization

Bischoff N, Raaf J, Olsen B, Bretner M, Issinger OG, Niefind K (2011) Enzymatic activity with an incomplete catalytic spine: insights from a comparative structural analysis of human CK2a and its paralogous isoform CK2a´. Mol Cell Biochem, in press. Download

Bischoff N, Olsen B, Raaf J, Bretner M, Issinger OG, Niefind K (2011) Structure of the human protein kinase CK2 catalytic subunit CK2a and interaction thermodynamics with the regulatory subunit CK2b. J Mol Biol, 407(1), 1-12. Download Abstract

Raaf J, Bischoff N, Klopffleisch K, Brunstein E, Olsen BB, Vilk G, Litchfield DW, Issinger OG, Niefind K (2011) Interaction between CK2a and CK2b, the subunits of protein kinase CK2: thermodynamics contributions of key residues on the CK2a surface. Biochemistry, 50(4):512-22. Download Abstract

Olsen BB, Guerra B, Niefind K, Issinger OG (2010) Structural basis of the constitutive activity of protein kinase CK2. Methods Enzymol, 484:515-29. Download Abstract

Niefind K, Issinger OG (2010) Conformational plasticity of the catalytic subunit of protein kinase CK2 and its consequences for regulation and drug design. Biochim Biophys Acta, 1804(3):484-92. Download Abstract

Niefind K, Raaf J, Issinger OG (2009) Protein kinase CK2 in health and disease: Protein kinase CK2: from structures to insights. Cell Mol Life Sci, 66(11-12):1800-16. Download Abstract

Raaf J, Issinger OG, Niefind K (2009) First inactive conformation of CK2 alpha, the catalytic subunit of protein kinase CK2. J Mol Biol, 386(5):1212-21. Download Abstract

Raaf J, Brunstein E, Issinger OG, Niefind K (2008) The interaction of CK2alpha and CK2beta, the subunits of protein kinase CK2, requires CK2beta in a preformed conformation and is enthalpically driven. Protein Sci, 17(12):2180-6. Download

Olsen BB, Larsen MR, Boldyreff B, Niefind K, Issinger OG (2008) Exploring the intramolecular phosphorylation sites in human Chk2. Mutat Res, 646(1-2):50-9. Download Abstract

Olsen BB, Rasmussen T, Niefind K, Issinger OG (2008) Biochemical characterization of CK2a and a´ paralogues and their derived holoenzymes: Evidence for the existence of a heterotrimeric CK2a´- holoenzyme forming trimeric complexes. Mol Cell Biochem, 316(1-2):37-47. Download Abstract

Raaf J, Issinger OG, Niefind K (2008) Insight from soft X-rays: the chlorine and sulphur sub.structures of a CK2alpha/DRB complex. Mol Cell Biochem, 316(1-2):15-23. Download Abstract

Raaf J, Klopffleisch K, Issinger OG, Niefind K (2008) The catalytic subunit of protein kinase CK2 structurally deviates from its maize homologue in complex with the nucleotide competitive inhibitor emodin. J Mol Biol, 377, 1-8. Download Abstract

Raaf J, Brunstein E, Issinger OG, Niefind K (2008) The CK2alpha/ CK2beta interface of human protein kinase CK2 harbors a binding pocket for small molecules. Chem Biol 15, 111-117. Download Abstract

Niefind K, Yde CW, Ermakova I, Issinger OG (2007) Evolved to be active: sulfate ions define substrate recognition sites of CK2alpha and emphasise its exceptional role within the CMGC family of eukaryotic protein kinases. J Mol Biol 370, 427-438. Download Abstract

Olsen BB, Boldyreff B, Niefind OG, Issinger OG (2006) Purification and characterization of the CK2alpha´-based holoenzyme, an isozyme of CK2alpha: a comparative analysis. Protein Expr Purifi 47, 651-661. Download Abstract

Rasmussen T, Skoeth IH, Jensen HH, Niefind K, Boldyreff B, Issinger OG (2005) Biochemical characterization of the recombinant human Drosophila homologues Timekeeper and Andante involved in the Drosophila circadian oscillator. Mol Cell Biochem 274, 151-161. Download Abstract

Ermakova I, Boldyreff B, Issinger B, Niefind K (2003) Crystal structure of a C-terminal deletion mutant of human protein kinase CK2 catalytic subunit. J Mol Biol 330, 925-934. Download Abstract

Niefind K, Guerra B, Ermakova I, Issinger OG (2001) Crystal structure of human protein kinase CK2: insights into basic properties of the CK2 holoenzyme. The EMBO J 20, 5320-31. Download Abstract

Grankowski N, Boldyreff B. Issinger OG (1991) Isolation and characterization of recombinant casein kinase II subunits alpha and beta from bacteria. Eur J Biochem 198, 25-30. Download Abstract

Antibodies against CK2 subunits

Schmidt-Spaniol I, Boldyreff B, Issinger OG (1992) Isolation and characterization of a monoclonal anti CK-2 alpha subunit antibody of the IgG1 subclass. Hybridoma 11, 53-59. Download Abstract

Nastainczyk W, Issinger OG, Guerra B (2003) Epitope analysis of the MAb 1AD9 antibody detection site in human protein kinase CK2alpha-subunit. Hybrid Hybridomics 22, 87-90. Download Abstract

Nastainczyk W, Schmidt-Spaniol I, Boldyreff B, Issinger OG (1995) Isolation and characterization of a monoclonal anti-protein kinase CK2beta-subunit antibody of the IgG class for the direct detection of CK2beta in tissue cultures of various mammalian species and human tumors. Hybridoma 14, 335-339. Download Abstract